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Online publication date: May An occlusion period of 1, 3, or 5 minutes with an inflatable cuff was used to create the FMD response. Online publication date: 4-Oct Use of a flow-mediated vasodilation technique to assess endothelial function in dogs. The 3-minute occlusion period provided equal or better responses than the 5-minute occlusion period. Change from baseline in luminal arterial diameter was most substantial when measured at 30 seconds following release of occlusion, whereas blood flow velocity changes were maximal when measured at 15 seconds following release.

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